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起止时间:2021-03-15到2021-06-30
更新状态:已完结
第四章 目的基因从哪来？ 第四章（文库、PCR）测试题
1、 用碱法分离质粒DNA时，染色体DNA之所以可以被除去，是因为：
A: 染色体DNA断成了碎片
B:染色体DNA分子量大，而不能释放
C:染色体变性后来不及复性
D:染色体未同蛋白质分开而沉淀
答案: 染色体变性后来不及复性
2、 Which of the following would be considered a transgenic organism?
A:a bacterium that has received genes via conjugation
B:a human given a corrected human blood-clotting gene
C:a fern grown in cell culture from a single fern root cell
D:a rat with rabbit hemoglobin genes
答案: a rat with rabbit hemoglobin genes
3、 重组DNA的筛选与鉴定不包括哪一方法
A:限制酶酶切图谱鉴定
B:PCR扩增鉴定
C:显微注射
D:蓝白筛选
答案: 显微注射
4、 ”Golden rice” was created by
A:manipulating the genome of a commonly-grown agricultural species of plant.
B:introduction of DNA from both prokaryotes （原核生物）and other eukaryotes （真核生物）not closely related to the rice plant.
C:a public facility, and distributed without commercial restraints.
D:None of the above.
答案: None of the above.
5、 In the polymerase chain reaction (PCR), the sequence of bases in the primers is important because it
A:determines which segment of the genome will be amplified
B:always matches a stop codon
C: always causes a silent mutation
D:determines how many cycles of the reaction are needed to obtain a sufficient amount of amplified DNA
答案: determines which segment of the genome will be amplified
6、 Human nerve cells differ from human muscle cells because different sets of genes are expressed; in each type of cell, different genes are transcribed into mRNA and translated into protein. Which of the following techniques would be the most efficient way to identify the genes that these cells express?
A: gel electrophoresis of DNA fragments
B:DNA microarray assays
C:isolating and analyzing all the proteins from each type of tissue
D:PCR
答案: PCR
7、 DNA synthesized using an RNA template is called
A:reverse transcriptase
B:a restriction enzyme
C:cDNA
D:a palindrome
答案: cDNA
8、 DNA probes are used to
A:locate complementary RNA sequences in a test sample
B: make homologous DNA
C:make homologous RNA
D: locate a specific DNA nucleotide sequence in a test sample
答案: locate a specific DNA nucleotide sequence in a test sample
9、 A DNA library is
A:a general collection of all genes sequenced thus far.
B:a collection of DNA fragments that make up the entire genome of a particular organism.
C: a DNA fragment inserted into a vector.
D: all DNA fragments identified with a probe.
答案: a collection of DNA fragments that make up the entire genome of a particular organism.
10、 A probe is used in which stage of genetic engineering?
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